General Cell Based Assays
Eurofins Discoverx PathHunter eXpress EphB3 Functional Assay, 10-Plate Kit. (93-0842E3CP16L)
The PathHunter eXpress EphB3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB3 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical FFAR1 (GPR40) Reporter Assay Kit, 1 ea
FFAR1/GPR40 is a G protein-coupled receptor for long-chain fatty acids such as DHA and EPA ω-3 fatty acids. It is involved in the regulation of insulin release from pancreatic β cells and mediates triglyceride-induced secretion of incretins GLP-1 and GIP from intestinal endocrine cells. Cayman’s FFAR1 (GPR40) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for FFAR1, an engineered G protein that directs Gαq activation signals to the Gαs pathway, and a cAMP response element-regulated SEAP reporter (FFAR1 reverse transfection strip plate). Cells grown on the transfection complex will express FFAR1 at the cell surface and the recombinant G protein inside the cell. Binding of agonists to FFAR1 initiates a signal transduction cascade resulting in expression of SEAP, which is secreted into the cell culture medium. Aliquots of culture medium are collected 16-24 hours after.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0640E2CP0L)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
BioVendor, LLC Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays), Multispecies colorimetric assay, Biocolor Item # S6000
Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays). The Sircol; INSOLUBLE Collagen Assay is a dye-binding method for analysis of insoluble collagen fibers. The assay be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent. Multispecies colorimetric assay. Detection Limit: 10.0 g. Assay-time: 4 hours. Biocolor Item # S6000
Abcam SIRT2 Activity Assay Kit (Fluorometric)
SIRT2 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Glycogen Phosphorylase Inhibitor, Purity: ≥98%, CAS: 648926-15-2, 1 mg
A cell-permeable acyl urea first identified as an inhibitor of human liver glycogen phosphorylase (IC50 = 53 nM); blocks glucagon-induced hepatic glycogenolysis in vivo
Eurofins Discoverx PathHunter eXpress TYK2 Activity Assay, 2-Plate Kit. (93-0875E3CP16M)
The PathHunter eXpress TYK2 Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the TYK kinase domain. The cell line is engineered to express the catalytic domain from TYK2 fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the TYK2 catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 2pl. (93-0999Y3-00053)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Thrombin Activity Assay Kit (Fluorometric)
Thrombin Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Glycine Assay Kit (Fluorometric)
Glycine Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Cell-Based Assay Buffer Tablet, 2 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Abcam LDH-Cytotoxicity Assay Kit (Fluorometric)
LDH-Cytotoxicity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery R-PLEX Rat IL-2 ASY
The R-PLEX Rat IL-2 Assay Kit from Meso Scale Discovery measures rat interleukin 2 (IL-2) in various biological fluids using MSD s electrochemiluminescent detection technology It offers high sensitivity a wide dynamic range and low sample volume requirements The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay quantitatively measures rat IL-2 crucial for the immune system
Eurofins Discoverx AssayComplete Cell Plating 21 Reagent, 500. (93-0563R21B)
AssayComplete Cell Plating 21 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress KDR Activity Assay, 2-Plate Kit. (93-0903E3CP16M)
The PathHunter KDR Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cell line overexpresses ProLink-tagged fusion of the KDR kinase domain with transmembrane and extracellular ligand binding domain of EGFR; and also an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.